Efficacy and Safety of Chinese Medicinal Prescriptions in Treatment of Acute Gouty Arthritis： A Network Meta-analysis / 中国实验方剂学杂志

ObjectiveIn the treatment of acute gouty arthritis （AGA）， western medicine is mostly used for anti-inflammatory and analgesic purposes to control the blood uric acid level， but some patients are still at risk of poor control and recurrent attacks. Chinese medicinal prescriptions， potent in resisting inflammation and relieving pain， are able to stabilize the blood uric acid level， reduce acute attacks， and improve the clinical efficacy of western medicine. However， there is a lack of evidence to support their use as evidence-based medicine. This study employed network Meta-analysis （NMA） to evaluate the efficacy and safety of common Chinese medicinal prescriptions in the treatment of AGA， aiming to provide evidence-based medical evidence for the clinical use of Chinese medicinal prescriptions in the treatment of AGA. MethodChinese and English databases were searched for prospective cohort studies and randomized controlled trials （RCTs） on Chinese medicinal prescriptions against AGA from database inception to December 1， 2022. Stata software and Review Manager were used for statistical analysis. ResultForty-four papers with 3 564 cases involved were included in the current NMA. In terms of reducing blood uric acid， the cumulative probability results showed that Mahuang Lianyao Chixiaodou Tang showed optimal efficacy （87.60%）. In terms of relieving joint pain， Danggui Niantongtang and Guizhi Shaoyao Zhimutang showed optimal efficacy （92.00% and 82.30%）. In terms of improving erythrocyte sedimentation rate （ESR）， Simiaowan was superior to other prescriptions （87.00%）. In terms of reducing C-reactive protein （CRP）， Simiaowan and Baihutang modified with Guizhitang showed superior efficacy （76.00% and 66.10%）. In terms of safety， except for the basic treatment group， Mahuang Lianyao Chixiaodou Tang had the lowest probability of adverse events， and Danggui Niantongtang had the highest probability of adverse reactions during treatment. According to the results of cluster analysis， Mahuang Lianyao Chixiaodou Tang and Simiaowan are effective and safe. ConclusionAccording to the results of NMA， Chinese medicinal prescriptions can assist in the treatment of AGA and improve the effectiveness of western medicine. For patients with AGA， clinicians can choose Mahuang Lianyao Chixiaodou Tang or Simiaowan as an auxiliary drug for routine western medicine treatment.

Preparation of a recombinant tumor-targeting ribosome inactivating protein luffin-α-NGR and evaluation of its antitumor activity / 生物工程学报

Loofah seeds ribosome inactivating protein luffin-α was fused with a tumor-targeting peptide NGR to create a recombinant protein, and its inhibitory activity on tumor cells and angiogenesis were assessed. luffin-α-NGR fusion gene was obtained by PCR amplification. The fusion gene was ligated with pGEX-6p-1 vector to create a recombinant plasmid pGEX-6p-1/luffin-α-NGR. The plasmid was transformed into E. coli BL21, and the target protein was isolated and purified by GST affinity chromatography. The luffin-α-NGR fusion gene with a full length of 849 bp was successfully obtained, and the optimal soluble expression of the target protein was achieved under the conditions of 16 ℃, 0.5 mmol/L IPTG after 16 h induction. SDS-PAGE and Western blotting confirmed the recombinant protein has an expected molecular weight of 56.6 kDa. Subsequently, the recombinant protein was de-tagged by precision protease digestion. The inhibitory effects of the recombinant protein on liver tumor cells HepG2 and breast cancer cells MDA-MB-231 were significantly stronger than that of luffin-α. The Transwell and CAM experiment proved that the recombinant protein luffin-α-NGR also had a significant inhibitory effect on tumor cells migration and neovascularization. The inhibitory activity on tumor cells and angiogenesis of the recombinant luffin-α-NGR protein lays a foundation for the development of subsequent recombinant tumor-targeting drugs.

Pharmacokinetic and pharmacodynamic analysis of ferulic acid-puerarin-astragaloside in combination with neuroprotective in cerebral ischemia/reperfusion injury in rats

Objective: To investigate the effects of the active ingredients combined therapy on inflammatory factors interleukin 1 beta (IL-1β) and neuropeptide Y (NPY) based onpharmacodynamics in rats. Methods: The animal model was built by transient middle cerebral artery occlusion (MCAO). The method for evaluating the concentrations of the FA-Pr-Al components in rat plasma was established by using HPLC and the expression levels of IL-1βand NPY were determined by ELISA. A new mathematics method of the trend of percentage rate of change (PRC) was used to assess the correlation between pharmacokinetics (PK) and pharmacodynamics (PD). Results: FA-Pr-Al in combination reduced neurological deficits, decreased infarct volume and inhibited the expression levels of IL-1βand NPY (all P<0.05)compared with the model group. FA, Pr and Al all displayed two compartment open models in rats. Clockwise hysteresis loops were obtained by time-concentration-effect curves. IL-1βand NPY level changes in the plasma followed an opposite trend to the plasma concentration tendency after Cmax was reached. Astragaloside’s PRC value was significantly higher than those of FA and puerarin between 120 to 180 min. Conclusions: The pharmacokinetics of FA-Pr-Al in combination were closely related its pharmacodynamics in treating ischemia/reperfusion injury, and the components of FA-Pr-Al may have a synergistic pharmacological effect. Astragaloside may play a more pronounced role in regulating IL-1毬and NPY levels comparedwith puerarin or FA.

The application of proximal femoral intramedullary nail with anti-rotation in old patients with intertrochanteric fractures / 中国基层医药

Objective To explore the effect of proximal femoral intramedullary nail with anti-rotation (PFNA) in old patients with intertrochanteric fractures.Methods 82 patients with proximal femoral fractures were randomly divided into the control group(41 cases) and the observation group(41 cases).The patients in the control group were operated through DHS steel plate,while the patients in the observation group were operated through PFNA.After followed-up for six months,the function of hip joint and quality of life (QOL) of patients were compared between two groups.Results There wasn't any serious complications in two groups.The excellent and good rate was 90.2％ in the observation group and 75.6％ in the control group,there was significant difference between the two groups (x2 =4.100,P ＜ 0.05).The QOL after operation was increased in two groups,and the QOL in the observation group was higher than that in the control group(t =4.752,P ＜ 0.01).Conclusion The proximal femoral intramedullary nail with anti-rotation in treatment of old patients with intertrochanteric fractures can not only raise surgical treatment,but also improve QOL.

Genetic Marker Study of Plasmid,Transposon and Integron in Multidrug-resistant Acinetobacter baumannii in Xinjiang / 中华医院感染学杂志

OBJECTIVE To know genetic marker existence condition of plasmid,transposon and integron in multidrug-resistant Acinetobacter baumannii in Xinjiang.METHODS The plasmid(traA,traF),transposon(tnpA,tnpU,merA) and integron(intⅠ1) in MDR-ABA 20 strains were studied.RESULTS From them 5 strains(25%) were tnpU positiye,4 strains(20%) were intⅠ1 positive,4 strains were tnpU and intⅠ1 simultaneously positive,and others were negative.CONCLUSIONS IntegronⅠexists extensively in A.baumannii in Xinjiang.Their multidrug resistance is correlated with transposon and integron carried by ABA.

Protective effect of 3'-methoxy puerarin on cerebral ischemic injury/ reperfusion in rats / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the effect of 3'-methoxy puerarin on cerebral infarction volume and free radical change of focal cerebral ischemia-reperfusion injury in rats and discuss the protective effect of 3'-methoxy puerarin on the cerebral ischemic/ reperfusion injury.</p><p><b>METHOD</b>The thread method was used to induce middle cerebral artery embolization, to establish the focal cerebral ischemia-reperfusion model. Rats were divided into five groups: the sham and model group, the two-dose group (5, 10 mg x kg(-1) x d(-1)) of 3'-methoxy puerarin and nimodipine group (5 mg x kg(-1) x d(-1)). The behavior changes and volume of cerebral infarction were observed, and the leves of SOD and the content of MDA were measured.</p><p><b>RESULT</b>3'-methoxy puerarin could significantly improve the symptoms of neurological deficit and reduce the infarct volume, and increased SOD activity and reduced the content of MDA of cortex in cerebral ischemia-reperfusion injury rat, the action of 10 mg x kg(-1) of 3'-methoxy puerarin is more remarkable.</p><p><b>CONCLUSION</b>3'-methoxy puerarin has protective effect on focal cerebral ischemia-reperfusion injury in rat.</p>

Antimicrobial Agents Including Cefmetazole Against Extended-spectrum Beta-lactamases-producing Enterobacteriaceae:An in vitro Susceptibility Investigation / 中华医院感染学杂志

OBJECTIVE To investigate in vitro activities of 12 antimicrobial agents including cefmetazole against extended-spectrum beta-lactamases-producing Escherichia coli(528 strains),Klebsiella pneumoniae(311 strains) and Proteus mirabilis(15 strains).METHODS They all collected from 15 teaching hospitals in China during 2005 and 2006 and included in the study.The levels of minimal inhibitory concentration(MIC) of 12 antimicrobial agents were determined by agar dilution method.WHONET 5.4 Software was used to analyze the data.RESULTS Against ESBLs-producing E.coli and ESBLs-producing K.pneumoniae,carbapenems were the most active antimicrobial agents(all 100.0% susceptible),followed by cephamycins(80.1-97.3%).Piperacillin/tazobactam(78.5-95.1%)showed a higher activity than cefoperazone/sulbactam(44.1-56.2%).The susceptible rate to ceftazidime against ESBLs-producing E.coli was remarkably higher than the other three cephalosporins,however the differences did not happen to ESBL-producing K.pneumoniae obviously.The susceptible rate to cefuroxime was below 1.6%.ESBLs-producing K.pneumoniae showed high sensitivity to carbapenems,cephamycins and ?-lactam/lactamase inhibitor combinations(all 100% susceptible),however the susceptible rates to cephalosporins were relatively lower.CONCLUSIONS Carbapenems and cephamycins remain the relatively high activity against ESBLs-producing Enterobacteriaceae.

Intramedullary nails for correction of deformity in the lower limbs in children with osteogenesis imperfecta / 中国矫形外科杂志

[Objective]To explore the application of the intramedullary nails for correction of deformity in the lower limbs and decrease the opportunity of refracture in children with osteogenesis imperfecta.[Method]From Jan.2000 to Aug.2004,8 patients(3 males and 5 females),with 13 femurs and 3 tibias totally,with deformity in lower limps were corrected by multi-osteotomy and fixed with intramedullary nails,2 for K-wires;3 for Ender nails;and 3 for flexible intramedullary nails.[Result]All deformities were perfectly corrected.All patients were available at final follow up,for 9 months to 60 months,mean 30 months.None of the complications occurred postoperatively,such as infection in the incision or pin tract,nonunion,and refracture.One patient can walk by a stick from wheel-chair help preoperatively and one K-wire was disrupted near the end.[Conclusion]Multi-osteotomy and fixed intramedullary nails,can correct the deformity in the lower limbs perfectly and decrease the opportunity of refracture in children with osteogenesis imperfecta,which has been proved to be a reliable method.

Detection of qacE△1-sul1 in Acinetobacter baumannii Isolated from Xinjiang and Its Clinical Significance / 中华医院感染学杂志

OBJECTIVE To study the antibiotic resistance gene of qacE△1-sul1 in Acinetobacter baumannii isolated from inpatients in Urumqi and the distribution and difference of qacE△1-sul1 genotype in Xinjiang. METHODS Bacterial strains were identified by system of API and antibiotic susceptibility test was detected by K-B and microdilution methods according CLSI.PCR was used to determine the gene of qacE△1-sul1 and compared with the sequences in GenBank. RESULTS Antibiotic resistance phenotypes showed the resistance rate to cefataxime and tetracycline was the highest(100.0%),but that to cefoperazone/sulbactam was the lowest.The positive rate of qacE△1-sul1 gene in A.baumannii was 52.2%. CONCLUSIONS High resistance in A.baumannii is found in Xinjiang.The positive rate of qacE△1-sul1 is high.Strengthening epidemiology surveillance of resistance carried by integron-carrying gene is important.

Genotyping of ?-Lactamases,Aminoglycoside Modifying Enzymes and Chlorhexidine-sulfanilamide from Acinetobacter baumannii / 中华医院感染学杂志

OBJECTIVE To investigate the coding genes of ?-lactamases,aminoglycoside modifying enzymes and the drug-resistant to chlorhexidine-sulfanilamide genes on 20 Acinetobacter baumannii isolates in Xinjiang.METHODS Twenty strains of A.baumannii were isolated from hospitalized patients,and 9 kinds of ?-lactamases genes,3 kinds of aminoglycoside modifying enzymes genes and drug-resistant to chlorhexidine-sulfanilamide genes were detected.The drug-resistant to chlorhexidine-sulfanilamide genes were labeled and cluster analysis was performed to analyze the affinity of strain.RESULTS The detection rates of ?-lactamases coding genes of TEM,ADC and SHV groups were 65%,60% and 5%,respectively.The others were not found in all 20 isolates tested.The detection rates of aminoglycoside modifying enzymes coding genes of aac(3)-Ⅰ,aac(6′)-Ⅰ and aac(3″)-Ⅰwere 60%,65%and 70%,respectively.And the detection rates of qacE△1-stull genes were 70%.There were 9 strains showed clone transmission according to cluster analysis.CONCLUSIONS Drug-resistance of the 20 strains to ?-lactam and aminoglycosides is connected with ?-lactamases and aminoglycoside modifying enzymes,and there exists clone transmission.

A rapid quantitative ELISA for measuring D-dimer to exclude pulmonary embolism / 中华检验医学杂志

494 ng/ml. The test showed a sensitivity of 100%, a negative predictive vale of 100%. Conclusion VIDAS D dimer is well sited as diagnostic tool for the exclusion pulmonary embolism.

Experimental study on the fatal infection with Vibrio vulnificus / 中华检验医学杂志

Objective To identify the histopathological characteristics of multiple organ dysfunction syndrome (MODS) with V. vulnificus in mice. Methods Sixteen healthy KM mice (6～8 week old ) divided randomly into two groups, study group ( n =12) and control group ( n =4) The animal model of MODS was established by received either an intraperitioneal, intramuscular subcuneous inoculum of 4.34?10 6 cfu/0.2 ml of V.vulnificus or intraperitioneal injection of a sterile physiological salt solution (control group). Pathological changes of the man organs were individually obsenved in under election microscope (EM). Results Mortality rates exced 100%, 12/12) in study group after inoculums of mice within 4～8 h, while in 0% (0/0) in the control . The detection rate of V. vulnificus were in 100%(12/12) from blood, hearts, lungs, livers, intramuscularly, subcutaneous in the study group, while in 0% (0/4) after sterile saline intraperitioneal injection. The man histopathological changes were :degeneration and necrosis of the parenchyma cells in the different organs;interstitial swelling, mitochondriondrial injure of multiple organs.These changes were especially obvious in the lungs and myocardeum. Conclusions Above pathological changes suggested that results of MODS caused by V. vulnificus septicemia,the multiple organs failure as an important feature of the fatality of V. vulnificus infections,and my be helpful for researchers investigating of V.vulnificus.

Analysis of 261 Cases With Colorectal Carcinoma / 中国医师杂志

Objective To explore the new feature of colorectal carcinoma attack and its clinical significance.Method The clinical data of the 261 cases of colorectal carcinoma were retrospectively analyzed in our hospital examined patients(from 1990 to 1998).Results The morbidity of colorectal carcinoma was rapid increased with time progression ,in the former four years was 28 7% ,but in the latter four years was increased to 71 3%, former four years median of attack age was 54 years,latter four years was 63 years.they had differential histological typing and clinical manifestation.Conclusion The colonoscope technique is helpful to the early differential dignosis of the colorectal carcinoma and colectal inflammatory enteropathy.